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KMID : 0364920080330030099
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Journal of Radiation Protection and Research
2008 Volume.33 No. 3 p.99 ~ p.104
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Representative Parameter of Immunostimulatory Ginseng Polysaccharide to Predict Radioprotection
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Son Hyeog-Jin
Shim Ji-Young
Ahn Ji-Yeon
Yun Yeon-Sook
Song Jie-Young
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Abstract
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According to the increase in the use of radiotherapy to cancer patients, many approaches have been tried to develop new agents for the protection of surrounding normal tissues. However, it is still few applied in the clinic as a radioprotector. We aim to find a representative parameter for radioprotection to easily predict the activity of in vivo experiment from the results of in vitro screening. The polysaccharide extracted from Panax ginseng was used in this study because the immunostimulator has been regarded as one of the radioprotective agent category and was already reported having a promising radioprotective activity through the increase of hematopoietic cells and the production of several cytokines. Mitogenic activity, AK cells activity and nitric oxide production were monitored for the in vitro immunological assay, and endogenous colony-forming unit (e-CFU) was measured as in vivo radioprotective parameter. The immunological activity was increased by the galactose contents of ginseng polysaccharide dependently. The result of this study suggests that mitogenic activity of splenocytes demonstrated a good correlation with in vivo radioprotective effect, and may be used as a representative parameter to screen the candidates for radioprotector.
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KEYWORD
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Immunostimulator, Mitogenic, Nitric oxide, Activated killer (AK) cells, Endogenous-colony forming unit (e-CFU)
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